08.10.2019
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A combination of new-generation DNA dyes, high-end instrumentation and sophisticated analysis software allows to detect these changes and to derive. The first multi-well HRM instruments were the Rotor-Gene 6000 (Corbett Life Science) and the LightScanner (Idaho Technology) (PDF). Download the HRM guide. We developed and validated a real-time quantitative polymerase chain reaction (qPCR) assay to determine Mycoplasma genitalium bacterial load in endocervical swabs, based on amplification of the pdhD gene which encodes dihydrolipoamide dehydrogenase, using the Rotor-Gene platform. We first determined the qPCR assay sensitivity, limit of detection, reproducibility and specificity, and then determined the ability of the qPCR assay to quantify M. Genitalium in stored endocervical specimens collected from Zimbabwean women participating in clinical research undertaken between 1999 and 2007. The qPCR assay had a detection limit of 300 genome copies/mL and demonstrated low intra- and inter-assay variability.
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The assay was specific for M. Genitalium DNA and did not amplify the DNA from other mycoplasma and ureaplasma species. We quantified M. Chimica Un Approccio Molecolare Pdf Merge. Genitalium in 119 of 1600 endocervical swabs that tested positive for M. Genitalium using the commercial Sacace M.
Genitalium real-time PCR, as well as 156 randomly selected swabs that were negative for M. Genitalium by the same assay. Genitalium loads ranged between. Post navigation.